Genotyping of B. licheniformis based on a novel multi-locus sequence typing (MLST) scheme.

ABSTRACT:

Background

Bacillus licheniformis has for many years been used in the industrial production of enzymes, antibiotics and detergents. However, as a producer of dormant heat-resistant endospores B. licheniformis might contaminate semi-preserved foods. The aim of this study was to establish a robust and novel genotyping scheme for B. licheniformis in order to reveal the evolutionary history of 53 strains of this species. Furthermore, the genotyping scheme was also investigated for its use to detect food-contaminating strains.

Results

A multi-locus sequence typing (MLST) scheme, based on the sequence of six house-keeping genes (adk, ccpA, recF, rpoB, spo0A and sucC) of 53 B. licheniformis strains from different sources was established. The result of the MLST analysis supported pr

SUBMITTER: Madslien EH 

PROVIDER: S-EPMC3492095 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature