Systematic determination of replication activity type highlights interconnections between replication, chromatin structure and nuclear localization.

ABSTRACT: DNA replication is a highly regulated process, with each genomic locus replicating at a distinct time of replication (ToR). Advances in ToR measurement technology enabled several genome-wide profiling studies that revealed tight associations between ToR and general genomic features and a remarkable ToR conservation in mammals. Genome wide studies further showed that at the hundreds kb-to-megabase scale the genome can be divided into constant ToR regions (CTRs) in which the replication process propagates at a faster pace due to the activation of multiple origins and temporal transition regions (TTRs) in which the replication process propagates at a slower pace. We developed a computational tool that assigns a ToR to every measured locus and determines its replication activity type (CTR vers

SUBMITTER: Farkash-Amar S 

PROVIDER: S-EPMC3492150 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature