Tetracycline inhibits local inflammation induced by cerebral ischemia via modulating autophagy.

ABSTRACT:

Background

Tetracycline exerts neuroprotection via suppressing the local inflammation induced by cerebral ischemia. However, the underlying mechanism is not completely clear.

Methodology/principal findings

The mRNA and protein expressions of tumor necrosis factor α and interleukin 6 and the number of activated microglia were measured to detect the inflammatory process in the ischemic hemisphere. The key proteins of nuclear factor kappa B pathway and the binding activity of nuclear factor kappa B were also measured. Two key components of autophagy, Beclin 1 and LC3, were detected by western blotting. Pretreatment with tetracycline inhibited the mRNA and protein expressions of tumor necrosis factor α and interleukin 6 and decreased the numbers of activated and phagocytotic mic

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC3492486 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature