Py2T murine breast cancer cells, a versatile model of TGFβ-induced EMT in vitro and in vivo.

ABSTRACT:

Introduction

Increasing evidence supports a role of an epithelial to mesenchymal transition (EMT) process in endowing subsets of tumor cells with properties driving malignant tumor progression and resistance to cancer therapy. To advance our understanding of the underlying mechanisms, we sought to generate a transplantable cellular model system that allows defined experimental manipulation and analysis of EMT in vitro and at the same time recapitulates oncogenic EMT in vivo.

Methodology/results

We have established a stable murine breast cancer cell line (Py2T) from a breast tumor of an MMTV-PyMT transgenic mouse. Py2T cells display a metastable epithelial phenotype characterized by concomitant expression of luminal and basal cytokeratins and sheet migration. Exposure of Py2T

SUBMITTER: Waldmeier L 

PROVIDER: S-EPMC3492491 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature