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Overexpression of ribosomal RNA in cumulus cells of patients with polycystic ovary syndrome.

ABSTRACT: OBJECTIVE: To evaluate the level of ribosomal RNA (rRNA) in cumulus cells (CCs) from women with polycystic ovary syndrome (PCOS) undergoing ICSI. MATERIALS AND METHODS: The study population included three healthy oocyte donors (control) and three patients with PCOS. RNA expression in CCs was assessed using quantitative real-time PCR assay to measure the pre-rRNA transcripts (5'ETS region), 18 S, 5.8 S and 28 S rRNA. RESULTS: The level of 18 S rRNA is 3.9 times higher (p = 0.036) and the level of 5.8 S rRNA is 2.9 times higher (p = 0.049) in CCs of PCOS patients than in CCs of healthy women. The fold change in expression of 28 S rRNA in CCs of PCOS patients also exceeds that in the control group, but did not reach a statistical significance (p = 0.342). CONCLUSION: Our observations support the idea that CCs of PCOS patients contain comparatively more ribosomes that CCs of healthy oocyte donors that may indicate a higher proliferation rate or up-regulated translation of protein factors in ??s of PCOS patients.

SUBMITTER: Polzikov M

PROVIDER: S-EPMC3492582 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

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Overexpression of ribosomal RNA in cumulus cells of patients with polycystic ovary syndrome.

Polzikov Mikhail M Yakovenko Sergey S Voznesenskaya Julia J Troshina Maria M Zatsepina Olga O

Journal of assisted reproduction and genetics 20120707 10

<h4>Objective</h4>To evaluate the level of ribosomal RNA (rRNA) in cumulus cells (CCs) from women with polycystic ovary syndrome (PCOS) undergoing ICSI.<h4>Materials and methods</h4>The study population included three healthy oocyte donors (control) and three patients with PCOS. RNA expression in CCs was assessed using quantitative real-time PCR assay to measure the pre-rRNA transcripts (5'ETS region), 18 S, 5.8 S and 28 S rRNA.<h4>Results</h4>The level of 18 S rRNA is 3.9 times higher (p = 0.03 ...[more]

PMID: 22772454

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Project description:Polycystic ovary syndrome (PCOS) is typically characterized by a polycystic ovarian morphology, hyperandrogenism, ovulatory dysfunction, and infertility. Furthermore, PCOS patients undergoing ovarian stimulation have more oocytes; however, the poor quality of oocytes leads to lower fertilization and implantation rates, decreased pregnancy rates, and increased miscarriage rates. The complex molecular mechanisms underlying PCOS and the poor quality of oocytes remain to be elucidated. We obtained matched oocytes and cumulus cells (CCs) from PCOS patients, compared them with age-matched controls, and performed RNA sequencing analysis to explore the transcriptional characteristics of their oocytes and CCs. Moreover, we validated our newly confirmed candidate genes for PCOS by immunofluorescence. Unsupervised clustering analysis showed that the overall global gene expression patterns and transposable element (TE) expression profiles of PCOS patients tightly clustered together, clearly distinct from those of controls. Abnormalities in functionally important pathways are found in PCOS oocytes. Notably, genes involved in microtubule processes, TUBB8 and TUBA1C, are overexpressed in PCOS oocytes. The metabolic and oxidative phosphorylation pathways are also dysregulated in both oocytes and CCs from PCOS patients. Moreover, in oocytes, differentially expressed TEs are not uniformly dispersed in human chromosomes. Endogenous retrovirus 1 (ERV1) elements located on chromosomes 2, 3, 4, and 5 are rather highly upregulated. Interestingly, these correlate with the most highly expressed protein-coding genes, including tubulin-associated genes TUBA1C, TUBB8P8, and TUBB8, linking the ERV1 elements to the occurrence of PCOS. Our comprehensive analysis of gene expression in oocytes and CCs, including TE expression, revealed the specific molecular features of PCOS. The aberrantly elevated expression of TUBB8 and TUBA1C and ERV1 provides additional markers for PCOS and may contribute to the compromised oocyte developmental competence in PCOS patients. Our findings may also have implications for treatment strategies to improve oocyte maturation and the pregnancy outcomes for women with PCOS.

Dataset Information

Overexpression of ribosomal RNA in cumulus cells of patients with polycystic ovary syndrome.

Publications

Overexpression of ribosomal RNA in cumulus cells of patients with polycystic ovary syndrome.

Similar Datasets

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