Intraoperative fluorescence imaging of peripheral and central nerves through a myelin-selective contrast agent.

ABSTRACT:

Purpose

Patients suffer from complications as a result of unintentional nerve damage during surgery. We focus on improving intraoperative visualization of nerves through the use of a targeted fluorophore and optical imaging instrumentation.

Procedure

A myelin-targeting fluorophore, GE3111, was synthesized, characterized for its optical and myelin-binding properties using purified myelin basic protein, and evaluated in mice. Additionally, a compact instrument was adapted to visualize nerves.

Results

GE3111 was synthesized using a versatile methodology. Its optical properties were sensitive to the local environment both in vitro and in vivo. Following intravenous injection, central and peripheral nerves were visualized, with the kinetics of nerve uptake modifiable depen

SUBMITTER: Cotero VE 

PROVIDER: S-EPMC3492698 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature