Endocytic tubules regulated by Rab GTPases 5 and 11 are used for envelopment of herpes simplex virus.

ABSTRACT: Enveloped viruses employ diverse and complex strategies for wrapping at cellular membranes, many of which are poorly understood. Here, an ultrastructural study of herpes simplex virus 1 (HSV1)-infected cells revealed envelopment in tubular membranes. These tubules were labelled by the fluid phase marker horseradish peroxidase (HRP), and were observed to wrap capsids as early as 2 min after HRP addition, indicating that the envelope had recently cycled from the cell surface. Consistent with this, capsids did not colocalise with either the trans-Golgi network marker TGN46 or late endosomal markers, but showed coincidence with the transferrin receptor. Virus glycoproteins were retrieved from the plasma membrane (PM) to label wrapping capsids, a process that was dependent on both dynamin and R

SUBMITTER: Hollinshead M 

PROVIDER: S-EPMC3492727 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature