Endothelial lipase mediates HDL levels in normal and hyperlipidemic rabbits.

ABSTRACT:

Aim

Existing evidence suggests that endothelial lipase (EL) plays an important role in high-densitylipoprotein (HDL) metabolism. Because rabbits are a useful animal model for the study of human lipid metabolism and atherosclerosis, we characterized rabbit EL (rEL) expression and investigated its relationship with plasma HDL levels in normal and hyperlipidemic rabbits.

Methods

We cloned the rEL cDNA and analyzed the EL tissue expression using Northern blotting, real-time RT-PCR, Western blotting, and in situ hybridization. We evaluated the effects of rEL antisense on plasma HDL levels.

Results

We found that rEL mRNA was highly expressed in cholesterol synthesis-related organs, including the liver, testis, and adrenal along with its expression in the lung, kidney, bone

SUBMITTER: Zhang J 

PROVIDER: S-EPMC3493565 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature