Macrophage migration inhibitory factor produced by the tumour stroma but not by tumour cells regulates angiogenesis in the B16-F10 melanoma model.

ABSTRACT:

Background

Macrophage migration inhibitory factor (MIF) has been proposed as a link between inflammation and tumorigenesis. Despite its potentially broad influence in tumour biology and prevalent expression, the value of MIF as a therapeutic target in cancer remains unclear. We sought to validate MIF in tumour models by achieving a complete inhibition of its expression in tumour cells and in the tumour stroma.

Methods

We used MIF shRNA-transduced B16-F10 melanoma cells implanted in wild-type and MIF-/- C57Bl6 mice to investigate the effect of loss of MIF on tumour growth. Cytokine detection and immunohistochemistry (IHC) were used to evaluate tumours ex vivo.

Results

Macrophage migration inhibitory factor shRNA inhibited expression of MIF protein by B16-F10 melanoma c

SUBMITTER: Girard E 

PROVIDER: S-EPMC3493755 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature