Patterns of genomic loss of heterozygosity predict homologous recombination repair defects in epithelial ovarian cancer.

ABSTRACT:

Background

Defects in BRCA1, BRCA2, and other members of the homologous recombination pathway have potential therapeutic relevance when used to support agents that introduce or exploit double-stranded DNA breaks. This study examines the association between homologous recombination defects and genomic patterns of loss of heterozygosity (LOH).

Methods

Ovarian tumours from two independent data sets were characterised for defects in BRCA1, BRCA2, and RAD51C, and LOH profiles were generated. Publically available data were downloaded for a third independent data set. The same analyses were performed on 57 cancer cell lines.

Results

Loss of heterozygosity regions of intermediate size were observed more frequently in tumours with defective BRCA1 or BRCA2 (P=10(-11)). The homo

SUBMITTER: Abkevich V 

PROVIDER: S-EPMC3493866 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature