Peptidomics of three Bothrops snake venoms: insights into the molecular diversification of proteomes and peptidomes.

ABSTRACT: Snake venom proteomes/peptidomes are highly complex and maintenance of their integrity within the gland lumen is crucial for the expression of toxin activities. There has been considerable progress in the field of venom proteomics, however, peptidomics does not progress as fast, because of the lack of comprehensive venom sequence databases for analysis of MS data. Therefore, in many cases venom peptides have to be sequenced manually by MS/MS analysis or Edman degradation. This is critical for rare snake species, as is the case of Bothrops cotiara (BC) and B. fonsecai (BF), which are regarded as near threatened with extinction. In this study we conducted a comprehensive analysis of the venom peptidomes of BC, BF, and B. jararaca (BJ) using a combination of solid-phase extraction and reverse

SUBMITTER: Tashima AK 

PROVIDER: S-EPMC3494202 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature