Gut microbiota-derived propionate reduces cancer cell proliferation in the liver.

ABSTRACT:

Background

Metabolites released by the gut microbiota may influence host metabolism and immunity. We have tested the hypothesis that inulin-type fructans (ITF), by promoting microbial production of short-chain fatty acids (SCFA), influence cancer cell proliferation outside the gut.

Methods

Mice transplanted with Bcr-Abl-transfected BaF3 cells, received ITF in their drinking water. Gut microbiota was analysed by 16S rDNA polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) and qPCR. Serum Short-chain fatty acids were quantified by UHPLC-MS. Cell proliferation was evaluated in vivo, by molecular biology and histology, and in vitro.

Results

Inulin-type fructans treatment reduces hepatic BaF3 cell infiltration, lessens inflammation and increases

SUBMITTER: Bindels LB 

PROVIDER: S-EPMC3494429 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature