Loop-mediated isothermal amplification for the detection of goose circovirus.

ABSTRACT:

Background

Goose circovirus (GCV) presents an immunosuppressive problem in production of geese. The infection's clinical symptoms include growth retardation or feathering disorders but the infection process may remain non-symptomatic what makes the infected birds more susceptible for secondary viral, bacterial and fungal infections. Diagnosis of GCV infection is made by histopathological examination, dot blot hybridization, polymerase chain reaction (PCR) and real-time PCR. However these techniques require application of thermocyclers and qualified staff which may be cost-consuming for some diagnostic units. The aim of this study was to develop loop-mediated isothermal amplification assay (LAMP) as a simple method of GCV detection.

Results

The presented study has shown LAMP

SUBMITTER: Wozniakowski G 

PROVIDER: S-EPMC3494565 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature