Using digital RNA counting and flow cytometry to compare mRNA with protein expression in acute leukemias.

ABSTRACT:

Background

The diagnosis of malignant hematologic diseases has become increasingly complex during the last decade. It is based on the interpretation of results from different laboratory analyses, which range from microscopy to gene expression profiling. Recently, a method for the analysis of RNA phenotypes has been developed, the nCounter technology (Nanostring® Technologies), which allows for simultaneous quantification of hundreds of RNA molecules in biological samples. We evaluated this technique in a Swiss multi-center study on eighty-six samples from acute leukemia patients.

Methods

mRNA and protein profiles were established for normal peripheral blood and bone marrow samples. Signal intensities of the various tested antigens with surface expression were similar to thos

SUBMITTER: Fernandez P 

PROVIDER: S-EPMC3494663 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature