A cell-based MHC stabilization assay for the detection of peptide binding to the canine classical class I molecule, DLA-88.

ABSTRACT: Identifying immunodominant CTL epitopes is essential for studying CD8+ T-cell responses in populations, but remains difficult, as peptides within antigens typically are too numerous for all to be synthesized and screened. Instead, to facilitate discovery, in silico scanning of proteins for sequences that match the motif, or binding preferences, of the restricting MHC class I allele - the largest determinant of immunodominance - can be used to predict likely candidates. The high false positive rate with this analysis ideally requires binding confirmation, which is obtained routinely by an assay using cell lines such as RMA-S that have defective transporter associated with antigen processing (TAP) machinery, and consequently, few surface class I molecules. The stabilization and resultant inc

SUBMITTER: Ross P 

PROVIDER: S-EPMC3494747 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature