Quantitative global and gene-specific promoter methylation in relation to biological properties of neuroblastomas.

ABSTRACT:

Background

In this study we aimed to quantify tumor suppressor gene (TSG) promoter methylation densities levels in primary neuroblastoma tumors and cell lines. A subset of these TSGs is associated with a CpG island methylator phenotype (CIMP) in other tumor types.

Methods

The study panel consisted of 38 primary tumors, 7 established cell lines and 4 healthy references. Promoter methylation was determined by bisulphate Pyrosequencing for 14 TSGs; and LINE-1 repeat element methylation was used as an indicator of global methylation levels.

Results

Overall mean TSG Z-scores were significantly increased in cases with adverse outcome, but were unrelated to global LINE-1 methylation. CIMP with hypermethylation of three or more gene promoters was observed in 6/38 tumors and 7

SUBMITTER: Kiss NB 

PROVIDER: S-EPMC3495052 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature