Selective labeling of polypeptides using protein farnesyltransferase via rapid oxime ligation.

ABSTRACT: An aldehyde-containing alternative substrate for protein farnesyltransferase was prepared and shown to be enzymatically incorporated into a peptide and a protein. The protein was subsequently immobilized onto aminooxy-functionalized agarose beads or labeled with a fluorophore. This method for protein modification provides an alternative to the commonly employed Cu(I)-catalyzed click reaction.

SUBMITTER: Rashidian M 

PROVIDER: S-EPMC3495172 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature