An improved allele-specific PCR primer design method for SNP marker analysis and its application.

ABSTRACT:

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Background

Although Single Nucleotide Polymorphism (SNP) marker is an invaluable tool for positional cloning, association study and evolutionary analysis, low SNP detection efficiency by Allele-Specific PCR (AS-PCR) still restricts its application as molecular marker like other markers such as Simple Sequence Repeat (SSR). To overcome this problem, primers with a single nucleotide artificial mismatch introduced within the three bases closest to the 3'end (SNP site) have been used in AS-PCR. However, for one SNP site, nine possible mismatches can be generated among the three bases and how to select the right one to increase primer specificity is still a challenge.

Results

In this study, different from the previous reports which used a limited quantity of pr

SUBMITTER: Liu J 

PROVIDER: S-EPMC3495711 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature