A putative role for amino acid permeases in sink-source communication of barley tissues uncovered by RNA-seq.

ABSTRACT:

Background

The majority of nitrogen accumulating in cereal grains originates from proteins remobilised from vegetative organs. However, interactions between grain filling and remobilisation are poorly understood. We used transcriptome large-scale pyrosequencing of flag leaves, glumes and developing grains to identify cysteine peptidase and N transporter genes playing a role in remobilisation and accumulation of nitrogen in barley.

Results

Combination of already known and newly derived sequence information reduced redundancy, increased contig length and identified new members of cysteine peptidase and N transporter gene families. The dataset for N transporter genes was aligned with N transporter amino acid sequences of rice and Arabidopsis derived from Aramemnon database. 57

SUBMITTER: Kohl S 

PROVIDER: S-EPMC3495740 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature