Development of 22 polymorphic microsatellite loci for the critically endangered Morato's Digger Toad, Proceratophrys moratoi.

ABSTRACT: The Morato's digger toad (Proceratophrys moratoi) inhabits Brazilian moist savannas and is critically endangered due to its very limited geographic distribution, reduced number of isolated populations, and evidence of population decline and local extinctions. With the objective of providing tools for the genetic study of the species, 22 polymorphic microsatellite loci were isolated and screened using DNA extracted from samples of oral mucosa cells obtained from 113 individuals representing five remnant P. moratoi populations in the Brazilian state of São Paulo. These markers presented 2-18 alleles per locus, polymorphism information content (PIC) of 0.02-0.87, observed heterozygosity of 0.02-0.96 and expected heterozygosity of 0.02-0.87. Three of the loci deviated significantly from Hardy-

SUBMITTER: Arruda MP 

PROVIDER: S-EPMC3497271 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature