Saliva samples are a viable alternative to blood samples as a source of DNA for high throughput genotyping.

ABSTRACT:

Background

The increasing trend for incorporation of biological sample collection within clinical trials requires sample collection procedures which are convenient and acceptable for both patients and clinicians. This study investigated the feasibility of using saliva-extracted DNA in comparison to blood-derived DNA, across two genotyping platforms: Applied Biosystems Taqman™ and Illumina Beadchip™ genome-wide arrays.

Method

Patients were recruited from the Pharmacogenetics of Breast Cancer Chemotherapy (PGSNPS) study. Paired blood and saliva samples were collected from 79 study participants. The Oragene DNA Self-Collection kit (DNAgenotek®) was used to collect and extract DNA from saliva. DNA from EDTA blood samples (median volume 8 ml) was extracted by Gen-Probe, Livingsto

SUBMITTER: Abraham JE 

PROVIDER: S-EPMC3497576 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature