Melting thermodynamics of reversible DNA/ligand complexes at interfaces.

ABSTRACT: A variety of solution methods exist for analysis of interactions between small molecule ligands and nucleic acids; however, accomplishing this task economically at the scale of hundreds to thousands of sequences remains challenging. Surface assays offer a prospective solution through array-based multiplexing, capable of mapping out the full sequence context of a DNA/ligand interaction in a single experiment. However, relative to solution assays, accurate quantification of DNA/ligand interactions in a surface format must contend with limited understanding of molecular activities and interactions at a solid-liquid interface. We report a surface adaptation of a solution method in which shifts in duplex stability, induced by ligand binding and quantified from melting transitions, are used for

SUBMITTER: Belozerova I 

PROVIDER: S-EPMC3498581 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature