Proteins differentially expressed in elicited cell suspension culture of Podophyllum hexandrum with enhanced podophyllotoxin content.

ABSTRACT:

Unlabelled

Background

Podophyllotoxin (PTOX), the precursor for semi-synthesis of cancer therapeutics like etoposide, teniposide and etophos, is primarily obtained from an endangered medicinal herb, Podophyllum hexandrum Royle. PTOX, a lignan is biosynthetically derived from the phenylpropanoid pathway. The aim of this study is to investigate changes in the P. hexandrum cell proteome potentially related to PTOX accumulation in response to methyl jasmonate (MeJA) elicitation. High-resolution two-dimensional gel electrophoresis (2-DE) followed by colloidal Coomassie staining and mass spectrometric analysis was used to detect statistically significant changes in cell's proteome.

Result

The HPLC analysis showed approximately 7-8 fold change in accumulation of PTOX, in th

SUBMITTER: Bhattacharyya D 

PROVIDER: S-EPMC3499389 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature