Identification of microRNAs from Eugenia uniflora by high-throughput sequencing and bioinformatics analysis.

ABSTRACT:

Background

microRNAs or miRNAs are small non-coding regulatory RNAs that play important functions in the regulation of gene expression at the post-transcriptional level by targeting mRNAs for degradation or inhibiting protein translation. Eugenia uniflora is a plant native to tropical America with pharmacological and ecological importance, and there have been no previous studies concerning its gene expression and regulation. To date, no miRNAs have been reported in Myrtaceae species.

Results

Small RNA and RNA-seq libraries were constructed to identify miRNAs and pre-miRNAs in Eugenia uniflora. Solexa technology was used to perform high throughput sequencing of the library, and the data obtained were analyzed using bioinformatics tools. From 14,489,131 small RNA clean reads,

SUBMITTER: Guzman F 

PROVIDER: S-EPMC3499529 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature