Application of pooled genotyping to scan candidate regions for association with HDL cholesterol levels.

ABSTRACT: Association studies are used to identify genetic determinants of complex human traits of medical interest. With the large number of validated single nucleotide polymorphisms (SNPs) currently available, two limiting factors in association studies are genotyping capability and costs. Pooled DNA genotyping has been proposed as an efficient means of screening SNPs for allele frequency differences in case-control studies and for prioritising them for subsequent individual genotyping analysis. Here, we apply quantitative pooled genotyping followed by individual genotyping and replication to identify associations with human serum high-density lipoprotein (HDL) cholesterol levels. The DNA from individuals with low and high HDL cholesterol levels was pooled separately, each pool was amplified by po

SUBMITTER: Hinds DA 

PROVIDER: S-EPMC3500196 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature