Production of four Neurospora crassa lytic polysaccharide monooxygenases in Pichia pastoris monitored by a fluorimetric assay.

ABSTRACT:

Unlabelled

Background

Recent studies demonstrate that enzymes from the glycosyl hydrolase family 61 (GH61) show lytic polysaccharide monooxygenase (PMO) activity. Together with cellobiose dehydrogenase (CDH) an enzymatic system capable of oxidative cellulose cleavage is formed, which increases the efficiency of cellulases and put PMOs at focus of biofuel research. Large amounts of purified PMOs, which are difficult to obtain from the native fungal producers, are needed to study their reaction kinetics, structure and industrial application. In addition, a fast and robust enzymatic assay is necessary to monitor enzyme production and purification.

Results

Four pmo genes from Neurospora crassa were expressed in P. pastoris under control of the AOX1 promoter. High yields

SUBMITTER: Kittl R 

PROVIDER: S-EPMC3500269 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature