Application of circular consensus sequencing and network analysis to characterize the bovine IgG repertoire.

ABSTRACT:

Background

Vertebrate immune systems generate diverse repertoires of antibodies capable of mediating response to a variety of antigens. Next generation sequencing methods provide unique approaches to a number of immuno-based research areas including antibody discovery and engineering, disease surveillance, and host immune response to vaccines. In particular, single-molecule circular consensus sequencing permits the sequencing of antibody repertoires at previously unattainable depths of coverage and accuracy. We approached the bovine immunoglobulin G (IgG) repertoire with the objective of characterizing diversity of expressed IgG transcripts. Here we present single-molecule real-time sequencing data of expressed IgG heavy-chain repertoires of four individual cattle. We describe the

SUBMITTER: Larsen PA 

PROVIDER: S-EPMC3500647 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature