Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research.

ABSTRACT: Microarrays are powerful tools for high throughput analysis, and hundreds or thousands of molecular interactions can be assessed simultaneously using very small amounts of analytes. Nucleotide microarrays are well established in plant research, but carbohydrate microarrays are much less established, and one reason for this is a lack of suitable glycans with which to populate arrays. Polysaccharide microarrays are relatively easy to produce because of the ease of immobilizing large polymers noncovalently onto a variety of microarray surfaces, but they lack analytical resolution because polysaccharides often contain multiple distinct carbohydrate substructures. Microarrays of defined oligosaccharides potentially overcome this problem but are harder to produce because oligosaccharides usually

SUBMITTER: Pedersen HL 

PROVIDER: S-EPMC3501085 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature