Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate.

ABSTRACT: Bioluminescence methodologies have been extraordinarily useful due to their high sensitivity, broad dynamic range, and operational simplicity. These capabilities have been realized largely through incremental adaptations of native enzymes and substrates, originating from luminous organisms of diverse evolutionary lineages. We engineered both an enzyme and substrate in combination to create a novel bioluminescence system capable of more efficient light emission with superior biochemical and physical characteristics. Using a small luciferase subunit (19 kDa) from the deep sea shrimp Oplophorus gracilirostris, we have improved luminescence expression in mammalian cells ~2.5 million-fold by merging optimization of protein structure with development of a novel imidazopyrazinone substrate (furim

SUBMITTER: Hall MP 

PROVIDER: S-EPMC3501149 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature