Effect of ligand density, receptor density, and nanoparticle size on cell targeting.

ABSTRACT: It is generally accepted that the presentation of multiple ligands on a nanoparticle (NP) surface can improve cell targeting; however, little work has been done to determine whether an optimal ligand density exists. We have recently developed a site-specific bioconjugation strategy that allows for distinct control of ligand density on a NP through the combined utilization of expressed protein ligation (EPL) and copper-free click chemistry. This EPL-Click conjugation strategy was applied to create superparamagnetic iron oxide (SPIO) NPs labeled with HER2/neu targeting affibodies at differing ligand densities. It was discovered that an intermediate ligand density provided statistically significant improvements in cell binding in comparison with higher and lower ligand densities. This interme

SUBMITTER: Elias DR 

PROVIDER: S-EPMC3502720 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature