Short-term stability of pathogen-specific nucleic acid targets in clinical samples.
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ABSTRACT:
SUBMITTER: Hasan MR
PROVIDER: S-EPMC3502979 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Publications
Short-term stability of pathogen-specific nucleic acid targets in clinical samples.
Hasan Mohammad R MR Tan Rusung R Al-Rawahi Ghada N GN Thomas Eva E Tilley Peter P
Journal of clinical microbiology 20121010 12
The stability of pathogen-specific DNA or RNA amplification targets in clinical samples following short-term storage at room temperature, 4°C, and -80°C was assessed by real-time PCR. In purified nucleic acid extracts, both DNA and RNA targets were stable for up to 30 days, irrespective of storage temperature. In unextracted samples, temperature-dependent loss of targets (P < 0.05) was observed in serum and cerebrospinal fluid specimens, while no changes were observed for EDTA blood samples. ...[more]
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