RNAi-mediated silencing of paramyosin expression in Trichinella spiralis results in impaired viability of the parasite.

ABSTRACT:

Background

Trichinella spiralis expresses paramyosin (Ts-PMY) not only as a structural protein but also as an immunomodulatory protein to protect the worm from being attacked by host complement components. In this study, the functions of PMY in the viability and the growth development of T. spiralis were confirmed at the first time by silencing the gene function with RNA interference technique.

Methods and findings

To understand its functions in the viability of the worm, we used RNA interference to silence the expression of Ts-pmy mRNA and protein in the parasite. Significant silencing of Ts-pmy mRNA expression in larval and adult T. spiralis was achieved by siRNA and dsRNA through soaking and electroporation. Electroporation of T. spiralis larvae with 8 µM siRNA1743 or 100

SUBMITTER: Chen X 

PROVIDER: S-EPMC3503832 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature