Molecular characterization of transgene integration by next-generation sequencing in transgenic cattle.

ABSTRACT: As the number of transgenic livestock increases, reliable detection and molecular characterization of transgene integration sites and copy number are crucial not only for interpreting the relationship between the integration site and the specific phenotype but also for commercial and economic demands. However, the ability of conventional PCR techniques to detect incomplete and multiple integration events is limited, making it technically challenging to characterize transgenes. Next-generation sequencing has enabled cost-effective, routine and widespread high-throughput genomic analysis. Here, we demonstrate the use of next-generation sequencing to extensively characterize cattle harboring a 150-kb human lactoferrin transgene that was initially analyzed by chromosome walking without success

SUBMITTER: Zhang R 

PROVIDER: S-EPMC3503979 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature