High-throughput discovery of chloroplast and mitochondrial DNA polymorphisms in Brassicaceae species by ORG-EcoTILLING.

ABSTRACT:

Background

Information on polymorphic DNA in organelle genomes is essential for evolutionary and ecological studies. However, it is challenging to perform high-throughput investigations of chloroplast and mitochondrial DNA polymorphisms. In recent years, EcoTILLING stands out as one of the most universal, low-cost, and high-throughput reverse genetic methods, and the identification of natural genetic variants can provide much information about gene function, association mapping and linkage disequilibrium analysis and species evolution. Until now, no report exists on whether this method is applicable to organelle genomes and to what extent it can be used.

Methodology/principal findings

To address this problem, we adapted the CEL I-based heteroduplex cleavage strategy used in

SUBMITTER: Zeng CL 

PROVIDER: S-EPMC3504036 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature