An L-glucose catabolic pathway in Paracoccus species 43P.

ABSTRACT:

Background

L-Glucose, the enantiomer of D-glucose, was believed not to be utilized by any organisms.

Results

An L-glucose-utilizing bacterium was isolated, and its L-glucose catabolic pathway was identified genetically and enzymatically.

Conclusion

L-Glucose was utilized via a novel pathway to pyruvate and D-glyceraldehyde 3-phosphate.

Significance

This might lead to an understanding of homochirality in sugar metabolism. An L-glucose-utilizing bacterium, Paracoccus sp. 43P, was isolated from soil by enrichment cultivation in a minimal medium containing L-glucose as the sole carbon source. In cell-free extracts from this bacterium, NAD(+)-dependent L-glucose dehydrogenase was detected as having sole activity toward L-glucose. This enzyme, LgdA, was purified, and

SUBMITTER: Shimizu T 

PROVIDER: S-EPMC3504760 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature