The first mammalian aldehyde oxidase crystal structure: insights into substrate specificity.

ABSTRACT:

Background

Aldehyde oxidases have pharmacological relevance, and AOX3 is the major drug-metabolizing enzyme in rodents.

Results

The crystal structure of mouse AOX3 with kinetics and molecular docking studies provides insights into its enzymatic characteristics.

Conclusion

Differences in substrate and inhibitor specificities can be rationalized by comparing the AOX3 and xanthine oxidase structures.

Significance

The first aldehyde oxidase structure represents a major advance for drug design and mechanistic studies. Aldehyde oxidases (AOXs) are homodimeric proteins belonging to the xanthine oxidase family of molybdenum-containing enzymes. Each 150-kDa monomer contains a FAD redox cofactor, two spectroscopically distinct [2Fe-2S] clusters, and a molybdenum cofactor

SUBMITTER: Coelho C 

PROVIDER: S-EPMC3504782 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature