Dynein light chain 1 (LC8) association enhances microtubule stability and promotes microtubule bundling.

ABSTRACT:

Background

Dynein Light Chain 1 (LC8) has been shown to pull down tubulin subunits, suggesting that it interacts with microtubules.

Results

LC8 decorates microtubules in vitro and in Drosophila embryos, promotes microtubule assembly, and stabilizes microtubules both in vitro and in tissue-cultured cells.

Conclusion

LC8 stabilizes microtubules.

Significance

Data provide the first evidence of a novel MAP-like function of LC8. Dynein light chain 1 (LC8), a highly conserved protein, is known to bind to a variety of different polypeptides. It functions as a dimer, which is inactivated through phosphorylation at the Ser-88 residue. A loss of LC8 function causes apoptosis in Drosophila embryos, and its overexpression induces malignant transformation of breast cancer c

SUBMITTER: Asthana J 

PROVIDER: S-EPMC3504791 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature