Transcriptome-scale homoeolog-specific transcript assemblies of bread wheat.

ABSTRACT:

Background

Bread wheat is one of the world's most important food crops and considerable efforts have been made to develop genomic resources for this species. This includes an on-going project by the International Wheat Genome Sequencing Consortium to assemble its large and complex genome, which is hexaploid and contains three closely related 'homoeologous' copies for each chromosome. This multi-national effort avoids the complications polyploidy entails for correct assembly of the genome by sequencing flow-sorted chromosome arms one at a time. Here we report on an alternate approach, a direct homoeolog-specific assembly of the expressed portion of the genome, the transcriptome.

Results

After assessment of the ability of various assemblers to generate homoeolog-specific assem

SUBMITTER: Schreiber AW 

PROVIDER: S-EPMC3505470 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature