Identification of yeast genes that confer resistance to chitosan oligosaccharide (COS) using chemogenomics.

ABSTRACT:

Background

Chitosan oligosaccharide (COS), a deacetylated derivative of chitin, is an abundant, and renewable natural polymer. COS has higher antimicrobial properties than chitosan and is presumed to act by disrupting/permeabilizing the cell membranes of bacteria, yeast and fungi. COS is relatively non-toxic to mammals. By identifying the molecular and genetic targets of COS, we hope to gain a better understanding of the antifungal mode of action of COS.

Results

Three different chemogenomic fitness assays, haploinsufficiency (HIP), homozygous deletion (HOP), and multicopy suppression (MSP) profiling were combined with a transcriptomic analysis to gain insight in to the mode of action and mechanisms of resistance to chitosan oligosaccharides. The fitness assays identified 39

SUBMITTER: Jaime MD 

PROVIDER: S-EPMC3505485 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature