The transcriptional landscape of the deep-sea bacterium Photobacterium profundum in both a toxR mutant and its parental strain.

ABSTRACT:

Background

The deep-sea bacterium Photobacterium profundum is an established model for studying high pressure adaptation. In this paper we analyse the parental strain DB110 and the toxR mutant TW30 by massively parallel cDNA sequencing (RNA-seq). ToxR is a transmembrane DNA-binding protein first discovered in Vibrio cholerae, where it regulates a considerable number of genes involved in environmental adaptation and virulence. In P. profundum the abundance and activity of this protein is influenced by hydrostatic pressure and its role is related to the regulation of genes in a pressure-dependent manner.

Results

To better characterize the ToxR regulon, we compared the expression profiles of wt and toxR strains in response to pressure changes. Our results revealed a complex exp

SUBMITTER: Campanaro S 

PROVIDER: S-EPMC3505737 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature