A model system for assessing and comparing the ability of exon microarray and tag sequencing to detect genes specific for malignant B-cells.

ABSTRACT:

Background

Malignant cells in tumours of B-cell origin account for 0.1% to 98% of the total cell content, depending on disease entity. Recently, gene expression profiles (GEPs) of B-cell lymphomas based on microarray technologies have contributed significantly to improved sub-classification and diagnostics. However, the varying degrees of malignant B-cell frequencies in analysed samples influence the interpretation of the GEPs. Based on emerging next-generation sequencing technologies (NGS) like tag sequencing (tag-seq) for GEP, it is expected that the detection of mRNA transcripts from malignant B-cells can be supplemented. This study provides a quantitative assessment and comparison of the ability of microarrays and tag-seq to detect mRNA transcripts from malignant B-cells. A mod

SUBMITTER: Kloster MB 

PROVIDER: S-EPMC3505742 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature