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Manipulation of gene expression in zebrafish using caged circular morpholino oligomers.

ABSTRACT: Morpholino oligomers (MOs) have been widely used to knock down specific genes in zebrafish, but their constitutive activities limit their experimental applications for studying a gene with multiple functions or within a gene network. We report herein a new design and synthesis of caged circular MOs (caged cMOs) with two ends linked by a photocleavable moiety. These caged cMOs were successfully used to photomodulate β-catenin-2 and no tail expression in zebrafish embryos.

SUBMITTER: Wang Y

PROVIDER: S-EPMC3505977 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Manipulation of gene expression in zebrafish using caged circular morpholino oligomers.

Wang Yuan Y Wu Li L Wang Peng P Lv Cong C Yang Zhenjun Z Tang Xinjing X

Nucleic acids research 20120922 21

Morpholino oligomers (MOs) have been widely used to knock down specific genes in zebrafish, but their constitutive activities limit their experimental applications for studying a gene with multiple functions or within a gene network. We report herein a new design and synthesis of caged circular MOs (caged cMOs) with two ends linked by a photocleavable moiety. These caged cMOs were successfully used to photomodulate β-catenin-2 and no tail expression in zebrafish embryos. ...[more]

PMID: 23002141

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