Nuclear factor (erythroid-derived 2)-like 2 regulates drug resistance in pancreatic cancer cells.

ABSTRACT:

Objective

To investigate the molecular basis of drug resistance in pancreatic cancer.

Methods

The expression of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) levels in pancreatic cancer tissues and cell lines was analyzed. Clinical relevance between Nrf2 activation and drug resistance was demonstrated by measuring cell viability after Nrf2 and adenosine 5'-triphosphate-binding cassette, subfamily G member 2 (ABCG2) regulation by overexpression or knock-down of these genes. Activity of ABCG2 was measured by Hoechst 33342 staining.

Results

Abnormally elevated Nrf2 protein levels were observed in pancreatic cancer tissues and cell lines relative to normal pancreatic tissues. Increasing Nrf2 protein levels either by overexpression of exogenous Nrf2 or by activating e

SUBMITTER: Hong YB 

PROVIDER: S-EPMC3506252 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature