Focal adhesion kinase is a phospho-regulated repressor of Rac and proliferation in human endothelial cells.

ABSTRACT: Focal adhesion kinase (FAK) is critically positioned to integrate signals from the extracellular matrix and cellular adhesion. It is essential for normal vascular development and has been implicated in a wide range of cellular functions including the regulation of cell proliferation, migration, differentiation, and survival. It is currently being actively targeted therapeutically using different approaches. We have used human endothelial cells as a model system to compare the effects of inhibiting FAK through several different approaches including dominant negatives, kinase inhibitors and shRNA. We find that manipulations of FAK signaling that result in inhibition of FAK 397 phosphorylation inhibit proliferation and migration. However, abolition of FAK expression using stable (shRNA) or tr

SUBMITTER: Bryant PW 

PROVIDER: S-EPMC3507225 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature