A modular cell-based biosensor using engineered genetic logic circuits to detect and integrate multiple environmental signals.

ABSTRACT: Cells perceive a wide variety of cellular and environmental signals, which are often processed combinatorially to generate particular phenotypic responses. Here, we employ both single and mixed cell type populations, pre-programmed with engineered modular cell signalling and sensing circuits, as processing units to detect and integrate multiple environmental signals. Based on an engineered modular genetic AND logic gate, we report the construction of a set of scalable synthetic microbe-based biosensors comprising exchangeable sensory, signal processing and actuation modules. These cellular biosensors were engineered using distinct signalling sensory modules to precisely identify various chemical signals, and combinations thereof, with a quantitative fluorescent output. The genetic logic ga

SUBMITTER: Wang B 

PROVIDER: S-EPMC3507625 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature