Transcriptome analysis of male and female Sebastiscus marmoratus.

ABSTRACT:

Background

The rockfish Sebastiscus marmoratus, which is widely distributed in the East Sea and the South Sea of China, is a sensitive model for the toxic effects and mechanisms of marine contaminants. To gain a global view of the molecular mechanism(s) whereby gene expression may influence sexual dimorphism in S. marmoratus, and to develop a database for further toxicological studies, we performed a large-scale transcriptome study.

Methodology/principal findings

The Illumina DNA sequencing platform was employed to obtain 27,559,578 and 25,821,126 reads from two cDNA libraries generated from adult male and female S. marmoratus, respectively. Transcriptome de novo assembly was carried out with the short reads assembling program-SOAPdenovo. The reads assembled into 78,675 unig

SUBMITTER: Sun L 

PROVIDER: S-EPMC3507777 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature