Mitochondria-localized glutamic acid-rich protein (MGARP) gene transcription is regulated by Sp1.

ABSTRACT:

Background

Mitochondria-localized glutamic acid-rich protein (MGARP) is a novel mitochondrial transmembrane protein expressed mainly in steroidogenic tissues and in the visual system. Previous studies showed that MGARP functions in hormone biosynthesis and its expression is modulated by the HPG axis.

Methodology/principal findings

By bioinformatics, we identified two characteristic GC-rich motifs that are located proximal to the transcription start site (TSS) of MGARP, and each contains two Specificity protein 1 (Sp1) binding elements. We then determined that the -3 kb proximal MGARP promoter is activated in a Sp1-dependent manner using reporter assays and knockdown of Sp1 led to decreased expression of endogenous MGARP messages. We also demonstrated that one of the two GC-r

SUBMITTER: Jin D 

PROVIDER: S-EPMC3507827 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature