Identification of the Streptococcus mutans LytST two-component regulon reveals its contribution to oxidative stress tolerance.

ABSTRACT:

Background

The S. mutans LrgA/B holin-like proteins have been shown to affect biofilm formation and oxidative stress tolerance, and are regulated by oxygenation, glucose levels, and by the LytST two-component system. In this study, we sought to determine if LytST was involved in regulating lrgAB expression in response to glucose and oxygenation in S. mutans.

Results

Real-time PCR revealed that growth phase-dependent regulation of lrgAB expression in response to glucose metabolism is mediated by LytST under low-oxygen conditions. However, the effect of LytST on lrgAB expression was less pronounced when cells were grown with aeration. RNA expression profiles in the wild-type and lytS mutant strains were compared using microarrays in early exponential and late exponential phase

SUBMITTER: Ahn SJ 

PROVIDER: S-EPMC3507848 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature