Comparison of oral microbiota in tumor and non-tumor tissues of patients with oral squamous cell carcinoma.

ABSTRACT:

Background

Bacterial infections have been linked to malignancies due to their ability to induce chronic inflammation. We investigated the association of oral bacteria in oral squamous cell carcinoma (OSCC/tumor) tissues and compared with adjacent non-tumor mucosa sampled 5 cm distant from the same patient (n = 10). By using culture-independent 16S rRNA approaches, denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing, we assessed the total bacterial diversity in these clinical samples.

Results

DGGE fingerprints showed variations in the band intensity profiles within non-tumor and tumor tissues of the same patient and among the two groups. The clonal analysis indicated that from a total of 1200 sequences characterized, 80 bacterial species/phylotypes were

SUBMITTER: Pushalkar S 

PROVIDER: S-EPMC3507910 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature