Novel biosensor-based microarray assay for detecting rs8099917 and rs12979860 genotypes.

ABSTRACT:

Aim

To evaluate a novel biosensor-based microarray (BBM) assay for detecting rs12979860 and rs8099917 genotypes.

Methods

Four probes specific for rs8099917C/T or rs12979860G/T detection and three sets of quality control probes were designed, constructed and arrayed on an optical biosensor to develop a microarray assay. Two sets of primers were used in a one tube polymerase chain reaction (PCR) system to amplify two target fragments simultaneously. The biosensor microarray contained probes that had been sequenced to confirm that they included the rs8099917C/T or rs12979860G/T alleles of interest and could serve as the specific assay standards. In addition to rehybridization of four probes of known sequence, a total of 40 clinical samples collected from hepatitis C seropositiv

SUBMITTER: Li PY 

PROVIDER: S-EPMC3508644 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature